Influence of neem leaf powder as a natural coagulant in MBR employed to treat dairy wastewater
Vpliv prahu iz listov neema kot naravnega koagulanta v MBR, uporabljenem za čiščenje odpadne vode iz mlekarn
- Avtorji: R. Prakash, S. Pauline, M. Mahalakshmi, P. Bhuvaneswari, Vijayalakshmi Ramalingam
- Citat: Acta hydrotechnica, vol. 38, no. 69, pp. 127-146, 2025. https://doi.org/10.15292/acta.hydro.2025.09
- Povzetek: V tej študiji se je odpadna voda iz mlekarne pred čiščenjem z membranskim bioreaktorjem (MBR) predhodno obdelala z uporabo prahu iz listov neema kot naravnega koagulanta. Analize z vrstično elektronsko mikroskopijo (SEM), rentgensko difrakcijo (XRD), Fourierjevo transformacijsko infrardečo spektroskopijo (FTIR) in energijsko disperzijsko rentgensko analizo (EDAX) so pokazale, da ima prah neema veliko površino, amorfno-kristalno strukturo ter funkcionalne skupine in elemente, ki spodbujajo koagulacijo. Testi v kozarcih so optimizirali odmerek koagulanta, pH, čas mešanja in trajanje usedanja. V idealnih pogojih sta se motnost in KPK znatno zmanjšala. Oba sistema MBR, s predhodno obdelavo z neemom in brez nje, sta bila testirana glede učinkovitosti zmanjšanja KPK, transmembranskega tlaka (TMP), permeatnega toka, specifične hitrosti privzema kisika (SOUR) in usedanja blata (SVI). V eksperimentalnih reaktorjih MBR je predhodna obdelava na osnovi neema bistveno povečala učinkovitost odstranjevanja KPK, ki je dosegla vrh pri ~99 % pri 6-urnem hidravličnem zadrževalnem času (HRT) v primerjavi s ~90 % brez predhodne obdelave. Sistem, integriran z neemom, je pokazal zmanjšano kopičenje TMP (17,9 kPa v primerjavi z 27,6 kPa), povečano zadrževanje toka (22,7 litra na kvadratni meter na uro (LMH) v primerjavi z 12,9 LMH) in okrepljeno mikrobno aktivnost, merjeno s hlapnimi suspendiranimi snovmi v mešanici aktivnega blata in vode (MLVSS) (23,5 mg O₂/g MLVSS/h). Ti rezultati kažejo, da lahko prah neema poveča biorazgradljivost in zmanjša mašenje membran, s čimer postane upravljanje odpadne vode iz mlekarn bolj trajnostno.
- Ključne besede: MBR, koagulant, prah listov neema, zmanjšanje KPK, transmembranski tlak, specifična hitrost privzema kisika, usedanje blata, mašenje.
- Polno besedilo: a38rp.pdf
- Viri:
- Afzal, Muhammad & Khan, Shahzad & Zeshan, Basit & Riaz, Muhammad & Ejaz, Umer & Saleem, Ayesha & Zaineb, Rida & Sindhu, Haseeb & Yean, Chan & Ahmed, Naveed. (2023). Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities. Molecules 28, 1743. https://doi.org/10.3390/molecules28041743.
- Alazaiza, M. Y. D., Albahnasawi, A., Ali, G. A. M., Bashir, M. J. K., Nassani, D. E., Al Maskari, T., Amr, S. S. A., & Abujazar, M. S. S. (2022). Application of Natural Coagulants for Pharmaceutical Removal from Water and Wastewater: A Review. Water 14(2), 140. https://doi.org/10.3390/w14020140.
- Albahnasawi, A., Ali, G. A. M., Bashir, M. J. K., Nassani, D. E., Al Maskari, T., Amr, S. S. A., & Abujazar, M. S. S. (2022). Application of Natural Coagulants for Pharmaceutical Removal from Water and Wastewater: A Review. Water, 14(2), 140. https://doi.org/10.3390/w14020140.
- Alguacil, F. J. (2024). Recent advances in indium recovery. Metals, 14(11), 1282. https://doi.org/10.3390/met14111282.
- Ali, S. K., Abdou, M. M., Emara, M. M., Farag, R. S., & Mubarak, M. F. (2025). Eco-friendly solutions: A comprehensive review of natural coagulants for sustainable water treatment. Environmental Geochemistry and Health, 47, 535. https://doi.org/10.1007/s10653-025-02803-3.
- Al-Tayawi, Aws & Sisay, Elias & Beszédes, Sándor & Kertész, Sz. (2023). Wastewater Treatment in the Dairy Industry from Classical Treatment to Promising Technologies: An Overview. Processes 11, 2133. https://doi.org/10.3390/pr11072133.
- andem, G., & Jabłońska-Czapla, M. (2024). Indium as an emerging contaminant: Occurrence and environmental impact. Archives of Environmental Protection, 50(3). https://doi.org/10.24425/aep.2024.151688.
- Andersa, K. N., Tamiru, M., Teka, T. A., Ali, I. M., Chane, K. T., Regasa, T. K., & Ahmed, E. H. (2024). Proximate composition, phytochemicals, and safety of neem leaf flour: A review. Food Science & Nutrition, 12, 6929–6937. https://doi.org/10.1002/fsn3.4336.
- Aragaw TA and Bogale FM (2023), Role of coagulation/flocculation as a pretreatment option to reduce colloidal/ bio-colloidal fouling in tertiary filtration of textile wastewater: A review and future outlooks. Front. Environ. Sci. 11,1142227. https://doi.org/10.3389/fenvs.2023.1142227.
- Badawi, A. K., Salama, R. S., & Mostafa, M. M. M. (2023). Natural-based coagulants/flocculants as sustainable market-valued products for industrial wastewater treatment: a review of recent developments. RSC advances 13(28), 19335–19355. https://doi.org/10.1039/d3ra01999c.
- Bahrodin, Muhammad & Zaidi, Nur Syamimi & Hussein, Norelyza & Sillanpää, Mika & Prasetyo, Dedy & Syafiuddin, Achmad. (2021). Recent Advances on Coagulation-Based Treatment of Wastewater: Transition from Chemical to Natural Coagulant. Current Pollution Reports 7. https://doi.org/10.1007/s40726-021-00191-7.
- Chen, Y., Jiang, X., Yang, M., & Wang, Z. (2025). Biotechnology revival: in situ sludge minimization in wastewater. Frontiers in microbiology, 16, 1603215. https://doi.org/10.3389/fmicb.2025.1603215.
- Choy, S. Y., Prasad, K. M. N., Wu, T. Y., & Ramanan, R. N. (2014). A review on common vegetables and legumes as promising plant-based natural coagulants. International Journal of Environmental Science and Technology, 11, 1733–1748. https://doi.org/10.1007/s13762-013-0386-0.
- Corral Bobadilla, M., Lostado Lorza, R., Escribano García, R., Somovilla Gómez, F., & Vergara González, E. P. (2019). Coagulation: Determination of key operating parameters by multi-response surface methodology using desirability functions. Water, 11(2), 398. https://doi.org/10.3390/w11020398.
- Cote, Pierre & Brink, David & Adnan, Ali. (2006). Pretreatment Requirements for Membrane Bioreactors. Proceedings of the Water Environment Federation 1846-1855. https://doi.org/10.2175/193864706783750169.
- Daverey, Achlesh & Pakshirajan, Kannan. (2011). Pretreatment of Synthetic Dairy Wastewater Using the Sophorolipid-Producing Yeast Candida bombicola. Applied biochemistry and biotechnology 163, 720-8. https://doi.org/10.1007/s12010-010-9077-y.
- El-Sonbati, Adel & Diab, M. & Morgan, Sh.M. & Abou-Dobara, Mohamed & El-Ghettany, A.A.. (2019). Synthesis, characterization, theoretical and molecular docking studies of mixed-ligand complexes of Cu(II), Ni(II), Co(II), Mn(II), Cr(III), UO2(II) and Cd(II). Journal of Molecular Structure 1200, 127065. https://doi.org/10.1016/j.molstruc.2019.127065.
- Formentini-Schmitt, Dalila & Alves, Álvaro & Veit, Marcia & Bergamasco, Rosângela & Vieira, Angelica & Fagundes-Klen, Márcia. (2023). Ultrafiltration Combined with Coagulation/Flocculation/Sedimentation Using Moringa oleifera as Coagulant to Treat Dairy Industry Wastewater. Water 224. https://doi.org/10.1007/s11270-013-1682-2.
- Gardana, Rivelino & Mikhael, Magenta & Maulidiany, Nopa & Wandi, Asep. (2025). Effect of Hydraulic Retention Time on Food Chain Reactor (FCR) Performance in Industrial Wastewater Treatment. Syntax Literate; Journal Ilmiah Indonesia. https://doi.org/10. 3165-3180. https://doi.org/10.36418/syntax-literate.v10i3.57832.
- Goldstein, J. I., Newbury, D. E., Michael, J. R., Ritchie, N. W. M., Scott, J. H. J., & Joy, D. C. (2018). Scanning electron microscopy and X-ray microanalysis (4th ed.). Springer. https://doi.org/10.1007/978-1-4939-6676-9.
- Hassanzadeh, A., Tanisali, E., Hassas, B. V., Karakaş, F., & Celik, M. S., (2016). Effect of particle morphology on coagulation of silica. In IMPC 2016 - 28th International Mineral Processing Congress (IMPC 2016 - 28th International Mineral Processing Congress; Vol. 2016-September.
- Jensen, H., Gaw, S., Lehto, N. J., Hassall, L., & Robinson, B. H. (2018). The mobility and plant uptake of gallium and indium. Chemosphere, 209, 675–684. https://doi.org/10.1016/j.chemosphere.2018.06.111.
- Kani, Mophin Kani & Anju,. (2016). Exploring The Use of Orange Peel And Neem Leaf Powder As Alternative Coagulant in Treatment of Dairy Wastewater.., International Journal of Scientific & Engineering Research 7 (4), 238-244.
- Khan, Q., Imran, U., Ullman, J. L., & Khokhar, W. A. (2023). Turbidity removal through the application of powdered azadirachta indica (neem) seeds. Journal of Engineering and Technology 42(1), 1. https://doi.org/10.22581/muet1982.2301.01.
- Koul, B., Bhat, N., Abubakar, M., Mishra, M., Arukha, A. P., & Yadav, D. (2022). Application of Natural Coagulants in Water Treatment: A Sustainable Alternative to Chemicals. Water, 14(22), 3751. https://doi.org/10.3390/w14223751.
- Li Z, Wang X, Alberdi A, Deng J, Zhong Z, Si H, Zheng C, Zhou H, Wang J, Yang Y, Wright A-DG, Mao S, Zhang Z, Guan L and Li G (2020). Comparative Microbiome Analysis Reveals the Ecological Relationships Between Rumen Methanogens, Acetogens, and Their Hosts. Front. Microbiol. 11:1311. https://doi.org/10.3389/fmicb.2020.01311.
- Li-Yih Lin, Jiun-Lin Horng, Chieh-An Cheng, Chun-Yung Chang, Bor-Wei Cherng, Sian-Tai Liu, Ming-Yi Chou. (2022), Sublethal ammonia induces alterations of emotions, cognition, and social behaviors in zebrafish (Danio rerio), Ecotoxicology and Environmental Safety 244,114058, https://doi.org/10.1016/j.ecoenv.2022.114058.
- Lwasa, A., Mdee, O. J., Ntalikwa, J. W., & Sadiki, N. (2024). Performance analysis of plant-based coagulants in water purification: A review. Discover Water, 4(108). https://doi.org/10.1007/s43832-024-00171-0.
- Maeda, Y. (2024). Fouling of Reverse Osmosis (RO) and Nanofiltration (NF) Membranes by Low Molecular Weight Organic Compounds (LMWOCs), Part 1: Fundamentals and Mechanism. Membranes 14(10), 221. https://doi.org/10.3390/membranes14100221.
- Mahapatra, S. S. (2025). Improving the efficiency and optimization of wastewater treatment using response surface methodology (RSM). Water Environment Journal, 39(1), Article e70020. https://doi.org/10.1111/wej.70020.
- Manea, E. E., & Bumbac, C. (2024). Sludge composting — Is this a viable solution for wastewater sludge management Water, 16(16), 2241. https://doi.org/10.3390/w16162241.
- Mohan, Siva & K, Vidhya & C.T., Sivakumar & M, Sugnathi & V, Shanmugavadivu & Devi, M.. (2019). Textile Waste Water Treatment by Using Natural Coagulant (Neem-Azadirachta India). International Research Journal of Multidisciplinary Technovation. 1. 636-642. https://doi.org/10.34256/irjmtcon90.
- Namdeti, R., Joaquin, A. A., Meka, U. R., Ali Azam Al Amri, M. A., & Kashoub, A. S. A. M. (2023). Biocoagulants as Ecofriendly Alternatives in the Dairy Wastewater Treatment. Advances in Research, 16–23. https://doi.org/10.9734/air/2023/v24i1929.
- Nasr, M., Islam, M., Shehata, S. (2021) Smart Healthcare in the Age of AI: Recent Advances, Challenges, and Future Prospects. IEEE Access, 9, 145248–145270.
- https://doi.org/10.1109/ACCESS.2021.3118960.
- Patel, S., Thakur, A. S., & Shah, D. L. (2021). Nutritional and mineral profiling of Azadirachta indica leaves collected from different agro-climatic regions of India. Journal of Food Composition and Analysis, 96, 103703. https://doi.org/10.1016/j.jfca.2020.103703.
- Qian, R., Li, Y., Liu, Y., Sun, N., Liu, L., Lin, X., & Sun, C. (2024). Integrated transcriptomic and metabolomic analysis reveals the potential mechanisms underlying indium-induced inhibition of root elongation in wheat plants. Science of the Total Environment, 908, 168477. https://doi.org/10.1016/j.scitotenv.2023.168477.
- Rahman, T. U., Roy, H., Islam, M. R., Naddeo, V., & Islam, M. S. (2023). Advancement of membrane bioreactor technology toward sustainable industrial wastewater treatment. Membranes, 13(2), 181. https://doi.org/10.3390/membranes13020181.
- Ramsuroop, J., Gutu, L., Ayinde, W. B., Basitere, M., & Manono, M. S. (2024). A Review of Biological Processes for Dairy Wastewater Treatment and the Effect of Physical Parameters Which Affect Their Efficiency. Water, 16(4), 537. https://doi.org/10.3390/w16040537.
- Reem M. El-taweel, Nora Mohamed, Khlood A. Alrefaey, Sh Husien, A.B. Abdel-Aziz, Alyaa I. Salim, Nagwan G. Mostafa, Lobna A. Said, Irene Samy Fahim, Ahmed G. Radwan,(2023), A review of coagulation explaining its definition, mechanism, coagulant types, and optimization models; RSM, and ANN, Current Research in Green and Sustainable Chemistry 6, 2023, 100358. https://doi.org/10.1016/j.crgsc.2023.100358.
- S, M., K, V., C.T, S., M, S., V, S., & M, D. (2019). Textile Waste Water Treatment by Using Natural Coagulant (Neem-Azadirachta India). International Research Journal of Multidisciplinary Technovation, 1(6), 636–642. https://doi.org/10.34256/irjmtcon9.
- Sara Esteki, Milad Karsaz, Burhan Ghofrani, Reza Yegani, Sima Majidi,2024,Combination of membrane bioreactor with chemical coagulation for the treatment of real pharmaceutical wastewater: Comparison of simultaneous and consecutive pretreatment of coagulation on MBR performance, Journal of Water Process Engineering, 60,105108. https://doi.org/10.1016/j.jwpe.2024.105108.
- Shi, Z., Usman, M., He, J., Chen, H., Zhang, S., & Luo, G. (2021). Combined microbial transcript and metabolic analysis reveals the different roles of hydrochar and biochar in promoting anaerobic digestion of waste activated sludge. Water research, 205, 117679. https://doi.org/10.1016/j.watres.2021.117679.
- Singh, S., Kumar, M., & Chauhan, J. S. (2020). Assessment of heavy metals in medicinal plant leaves grown in urban and peri-urban areas of India. Environmental Science and Pollution Research, 27, 14721–14733. https://doi.org/10.1007/s11356-020-07976-8.
- Sirwan Alimoradi , Rasha Faraj , Ali Torabian. (2018). Effects of residual aluminum on hybrid membrane bioreactor (Coagulation-MBR) performance, treating dairy wastewater, Chemical Engineering and Processing - Process Intensification. 133, 320-324. https://doi.org/10.1016/j.cep.2018.09.023.
- Sisay, E. J., Al-Tayawi, A. N., László, Z., & Kertész, S. (2023). Recent Advances in Organic Fouling Control and Mitigation Strategies in Membrane Separation Processes: A Review. Sustainability 15(18), 13389. https://doi.org/10.3390/su151813389.
- Tahraoui, H., Toumi, S., Boudoukhani, M., Touzout, N., Sid, A. N. E. H., Amrane, A., Belhadj, A.-E., Hadjadj, M., Laichi, Y., Aboumustapha, M., Kebir, M., Bouguettoucha, A., Chebli, D., Assadi, A. A., & Zhang, J. (2024). Evaluating the Effectiveness of Coagulation–Flocculation Treatment Using Aluminum Sulfate on a Polluted Surface Water Source: A Year-Long Study. Water 16(3), 400. https://doi.org/10.3390/w16030400.
- Tsoutsa, E. K., Tolkou, A. K., Kyzas, G. Z., & Katsoyiannis, I. A. (2024). New Trends in Composite Coagulants for Water and Wastewater Treatment. Macromol 4(3), 509-532. https://doi.org/10.3390/macromol4030030.
- Wylie, M. R., & Merrell, D. S. (2022). The Antimicrobial Potential of the Neem Tree Azadirachta indica. Frontiers in pharmacology 13, 891535. https://doi.org/10.3389/fphar.2022.891535.
- Yin, Q., He, K., Collins, G. et al. Microbial strategies driving low concentration substrate degradation for sustainable remediation solutions. npj Clean Water 7, 52 (2024). https://doi.org/10.1038/s41545-024-00348-z.
- Zielińska, M., Galik, M. Use of Ceramic Membranes in a Membrane Filtration Supported by Coagulation for the Treatment of Dairy Wastewater. Water Air Soil Pollut 228, 173 (2017). https://doi.org/10.1007/s11270-017-3365-x.